Juvenile rheumatological patients were recruited from KK Women’s and Children’s Hospital with Institutional Review Board approval. Clinical data were collected blind to laboratory researchers (Supplementary Table 2). Blood (2 to 5 ml) was collected with consent into the Vacutainer Plus serum tubes (BD Biosciences, Franklin Lakes, NJ, USA) and, after clotting for 30 min, sera were harvested and stored at −80 °C.
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