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One-Step Zirconia-Silica Beads Method (ZSB)

YN Ya-Ting Ning
WY Wen-Hang Yang
WZ Wei Zhang
MX Meng Xiao
YW Yao Wang
JZ Jing-Jia Zhang
GZ Ge Zhang
SD Si-Meng Duan
AD Ai-Ying Dong
DG Da-Wen Guo
GZ Gui-Ling Zou
HW Hai-Nan Wen
YG Yan-Yan Guo
LC Li-Ping Chen
MC Miao Chai
JH Jing-Dong He
QD Qiong Duan
LZ Li-Xia Zhang
LZ Li Zhang
YX Ying-Chun Xu
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Rapid extraction of protein using the ZSB method was performed using the same cultures as the routine method. Approximately 1–2 cm2 pieces of mold were removed from the agar and added to a 1.5 ml tube containing 30 μl of zirconia-silica beads with a diameter of 0.5 mm and 60 ul extraction solution (consisting of 30 ul acetonitrile and 30 ul FA). The tubes were vortexed for 5 min at RT, and then centrifuged for 1 min at 13,800 g.

Copyright and License information:  ©2021 Ning, Yang, Zhang, Xiao, Wang, Zhang, Zhang, Duan, Dong, Guo, Zou, Wen, Guo, Chen, Chai, He, Duan, Zhang, Zhang and Xu
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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