Determination of the Minimum Inhibitory Concentration of Selected Compounds

WZ Wei Zhou
BY Bing Yang
YZ Yanyan Zou
KR Khaista Rahman
XC Xiaojian Cao
YL Yingying Lei
RL Ren Lai
ZF Zhen F. Fu
XC Xi Chen
GC Gang Cao
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For minimum inhibitory concentration (MIC) analysis, 100 μl of the bacterial suspension was prepared as above and added to the wells of a 96-well plate. Ten microliters of twofold serial dilutions of screened drugs (from 0.012 to 12.8 μg/ml) and peptides (from 1 to 512 μg/ml) was added to the indicated well. Bacterial growth was assessed visually after culture for 1 week.

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