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WAX-S tips extraction

RG Roberta Gorziza
JC Joseph Cox
RL Renata Pereira Limberger
LA Luis E. Arroyo-Mora
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Into a 1.5 mL polypropylene tube, 50 µL of oral fluid spiked with THC and CBD (at the concentration of 12 ng/mL), 100 µL of acetonitrile and 5 µL of an internal standard solution (1 µg/mL solution of THC-d3 and CBD-d3, with a final concentration of 50 ng/mL) were added. Using a 300 µL WAX-S tip and a micropipette, the mix was aspirated and dispensed for three times. Then, 100 µL of the top layer, a hydrophobic phase containing the analytes, was transferred to a glass vial and 1 µL of sample was injected in the LC–MS/MS for analysis.

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