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Cell migration assay

MW Ming-Heng Wu
YC Yuh-Ling Chen
KL Kuen-Haur Lee
CC Che-Chang Chang
TC Tsai-Mu Cheng
SW Szu-Yuan Wu
CT Chao-Chiang Tu
WT Wan-Lin Tsui
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Cell migration assays were done in 24-well transwell polycarbonate filters (pore size, 8 mm; Corning Costar). Briefly, LX-2 cells were seeded in the upper chamber, and chemoattractants such as Gal-1, PDGF, and TGF-β were added to the lower chamber. Migrated cells were counted after cells had been individually incubated for 24 h. Non-penetrating cells were removed from the upper surface of the filter with a cotton swab. Penetrating cells were fixed and stained with a DiffQuick stain kit (Dade Behring) according to the manufacturer’s instructions. For quantification, all of the cells that had migrated onto the lower surface were stained and counted under a light microscope. All experiments were performed in duplicate. Results are shown as the mean ± standard error of the mean (SEM) of three independent assays.

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