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Lactate Dehydrogenase (LDH) Release Assay
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Increased release of LDH indicated the loss of plasma membrane integrity. Briefly, cells were grown in a 96-well plate for various treatments. Cell supernatant was harvested at the indicated time point, and LDH release was examined with an LDH assay kit (Beyotime, China) at absorbance of 490 nm with a microplate reader. The level of LDH release was counted as the percentage of the total amount, considered as the sum of the enzymatic activity present in the cellular lysate and that in the cell supernatant.
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