2.7. Imaging of Fluorescent E. coli under Confocal Microscopy

The E. coli cells transformed with recombinant plasmids were incubated on poly-L-lysine coated slide glasses for 1 h and mounted sample using Antifade Mounting Medium with DAPI and FITC (Thermo Fisher Scientific). We examined the single cell image of the fluorescent E. coli with a Zeiss LSM 880 confocal laser-scanning microscope (Carl Zeiss, Oberkochen, Germany) and Zen^® Blue edition software (Zeiss).