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4.11.3. Determination of Protein Carbonyl Content
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A protein carbonyl content assay kit was used to measure protein oxidation according to the manufacturer’s protocol (abcam). The assay is based on the reaction of dinitrophenylhydrazine (DNPH) with protein carbonyls. The OD was measured at ~375 nm on a microplate reader (Pharmaspec). The obtained value was expressed as nmol/mg.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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