Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Brassica juncea Transformation

SA Sajad Ali
ZM Zahoor A. Mir
AT Anshika Tyagi
HM Hailay Mehari
RM Rajendra P. Meena
JB Javaid A. Bhat
PY Prashant Yadav
PP Pradeep Papalou
SR Sandhya Rawat
AG Anita Grover
request Request a Protocol
ask Ask a question
Favorite

Brassica juncea cv. Varuna seeds were germinated on half strength Murashige and Skoog (MS) medium in Magenta boxes (Magenta vessel Corp., United States) at 24 ± 2°C under cool white florescent light (90–150 μmol photons/m2s) in a 16/8 h (light/dark) photoperiod (Murashige and Skoog, 1962). BjNPR1 transgenic plants were generated through Agrobacterium mediated co-cultivation method according to protocol with some modifications (Sharma et al., 2009). Seeds obtained from T0 transformed B. juncea plants were primary screened on kanamycin selection medium and then transferred into pots for further analysis.

Copyright and License information:  ©2017 Ali, Mir, Tyagi, Mehari, Meena, Bhat, Yadav, Papalou, Rawat and Grover.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A