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2.11. Plasmatic 8-Hydroxy-2-Deoxyguanosine Quantification

AG Ana Cristina Gonçalves
RA Raquel Alves
IB Inês Baldeiras
BM Bárbara Marques
BO Bárbara Oliveiros
AP Amélia Pereira
JC José Manuel Nascimento Costa
EC Emília Cortesão
LV Luisa Mota Vieira
AR Ana Bela Sarmento Ribeiro
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The plasmatic 8-OHdG levels were measured using a competitive quantitative ELISA Kit (8-hydroxy-2-deoxyguanosine ELISA Kit, Abcam (Cambridge, UK)), according to the manufacturer’s instructions. The assay is based on the competition between 8-OHdG and an 8-OHdG acetylcholinesterase conjugate for a limited amount of 8-OHdG monoclonal antibody. The colorimetric intensity was determined spectrophotometrically in a SynergyTM multimode microplate reader, and its value was inversely proportional to the amount of free 8-OHdG in plasma.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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