Genetic knockdown using siRNA

DL David S. Liu
CD Cuong P. Duong
SH Sue Haupt
KM Karen G. Montgomery
CH Colin M. House
WA Walid J. Azar
HP Helen B. Pearson
OF Oliver M. Fisher
MR Matthew Read
GG Glen R. Guerra
YH Ygal Haupt
CC Carleen Cullinane
KW Klas G. Wiman
LA Lars Abrahmsen
WP Wayne A. Phillips
NC Nicholas J. Clemons
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Cells were reverse transfected with 40 nM p53, NRF2, SLC7A11 or non-targeting control siRNA pools (siGenome Smartpool, Dharmacon) using Lipofectamine RNAiMax solution (Life Technologies) according to the manufacturer's guidelines. Knockdown efficiency was assessed by RT-PCR and western blotting. siRNA sequences are detailed in Supplementary Table 5.

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