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Pseudo-temporal ordering of cells using Monocle 3
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The integrated data of Seurat were transferred to Monocle 3 (Trapnell et al., 2014, https://github.com/cole-trapnell-lab/monocle3, Trapnell et al., 2021), to calculate a cell trajectory using the learn_graph function. We assigned the start point based on the expression of cell proliferation-related genes and Drosophila marker genes of the cell cycle.
Copyright and License information: ©2021, Koiwai et al
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