Cell viability assay

AML cell lines and primary AML samples were seeded in a 96-well plate at a density of 8000-10,000 or 20,000-30,000 cells/well, respectively, and incubated with increasing concentrations of CPI-637, NEO1132, or NEO2734 for 96 hours. 10 µL MTT reagent (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; Sigma Aldrich) was added and cells were incubated for 4 (AML cell lines) or 6 hours (primary samples). Subsequently, MTT crystals were dissolved using 150 µL isopropanol-HCl and absorbance was measured at 560 nm using a GloMax Discover Microplate reader (Promega). Values are represented as percentages, using the formula optical density (OD) = (treated sample/OD untreated sample) × 100.