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Mouse islets were incubated in 10% FBS RPMI1640 medium with 5 μg/mL of tunicamycin (MP Biomedical) for 6 hours. INS1 cells were incubated in INS1 medium with 1 μg/mL of tunicamycin for 6 hours. Human islets were incubated in 1% HSA CMRL1066 medium with 10 μg/mL tunicamycin overnight prior to harvest.
Copyright and License information: ©2021 Mishra et al.
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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