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Zebrafish experiments

IS Iliana Serifi
SB Simoni Besta
ZK Zoe Karetsou
PG Panagiota Giardoglou
DB Dimitris Beis
PN Pawel Niewiadomski
TP Thomais Papamarcaki
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Breeding and maintenance of wild-type zebrafish and 12xGliBS:mCherry-NLS transgenic line were carried out as previously described36 in accordance with the European Directive 2010/63 for the protection of animals used for scientific purposes and the Recommended Guidelines for Zebrafish Husbandry64 . The experimental protocols described in this study were carried out with zebrafish larvae up to 48 h post fertilization and were approved by the Foundation for Research and Technology-Hellas (FORTH) Ethics Committee. Reverse genetics experiments involving CRISPR/Cas9 were performed and analyzed during embryonic stages of zebrafish development, as described36. The study was carried out in compliance with the ARRIVE guidelines (https://arriveguidelines.org).

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