2.5. Islet reaggregation

Isolated islets were dispersed with 0.25% trypsin and seeded into GravityPLUS plates (InSphero) at a density of 2000 cells per hanging drop. Aggregation was allowed to occur for 4 days, at which point reaggregated islets were dropped into recipient low-attachment wells. Insulin secretion assays and imaging were performed on the same day or 1 day after reaggregated islets were dropped. For adenovirus-treated reaggregated islets, dispersed islets were reaggregated in the presence of adenovirus particles mixed into the media used for hanging drop formation (MOI 200).