HTL epitopes were predicted using the IEDB MHCII server. Immune Epitope Database and Analysis Resources (IEDB) is an online database and suite to predict immune epitopes from an extensive collection of experimentally derived immune epitopes31. The amino acid sequences of the selected proteins were submitted to the server independently in fasta format. The default (IEDB recommended 2.22) setting was selected for predicting the epitopes utilizing the full HLA human reference set. The epitope length was specified to be15 mer and results were sorted according to their adjusted ranks.
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