Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Genetic characterization of TaAREB3

JW Jingyi Wang
QL Qian Li
XM Xinguo Mao
AL Ang Li
RJ Ruilian Jing
request Request a Protocol
ask Ask a question
Favorite

To analyze the genomic origin of TaAREB3 genome-specific sequences of TaAREB3 were amplified using the wheat diploid progenitor species. DNA fragments were ligated into a pEASY-Blunt cloning vector, and the vectors were transformed into E. coli; plasmids were extracted and sequenced with a DNA Analyzer ABI 3730. Diversity of the sequencing results was analyzed with the MegAlign program in DNAStar software.

Copyright and License information:  ©2016 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A