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Ethoxyresorufin-O-deethylase (EROD) and alanine aminotrasferase assays
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Ethoxyresorufin O-deethylase (EROD) activities were measured using different subcellular fractions from TCDD-treated and control liver tissues from both WT and RD7 mice. The reaction mixture contains 25 μg protein with 0.1 M NADPH, 7-ethoxyresorufin (approximately 0.1 mg/mL) and Tris-NaCl (TN) buffer (0.05 M Tris, pH 7.6, containing 0.1 M NaCl). Each reaction was performed as previously described57. The alanine aminotrasferase (ALT) assay was performed according to the manufacturer’s protocol (BioVision, Inc., Milpitas, CA).
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