2.7. Acute toxicity test

An acute oral toxicity study was performed according to the Organization for Economic Cooperation and Development guidelines 423 on limit toxicity testing.²¹ Five mice were used for the study with one of the mice serving as the control. The animals were fasted overnight with access to drinking water before the test. Each mouse received 2000 mg/kg of the ethanol extract, or for the control, distilled water (10 ml/kg). The mice were continuously observed during the first 4 h. Afterward, they were observed periodically for up to 24 h for mortality and other signs of toxicity and observed daily thereafter for 14 days.