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Escherichia coli, ATCC 25922 and ATCC 35218
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These strains were modified to overexpress the gene for the superfolder green fluorescent protein (sfGFP). This provides the possibility to monitor changes in cell viability and morphology induced by the presence of antibiotic compounds. We transformed these strains by electroporation42 with the plasmid pSEVA271_sfgfp (lab collection) carrying the sfgfp gene, which was constructed based on plasmid pSEVA27143 (kanamycin resistance) adding a gene for sfGFP44 under control of a constitutive promoter (BioBrick part BBa_J23100).45
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