4.8. ELISA Assay for Quantification of Cytokine Expression

RM Rui Medeiros
BH Bruno Horta
JF Joana Freitas-Silva
JS Jani Silva
FD Francisca Dias
ES Emília Sousa
MP Madalena Pinto
FC Fátima Cerqueira
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The quantification of cytokines (IL-1β, TNF-α, IL-10, and TGF-β1) was performed on conditioned macrophage supernatants using uncoated ELISA kits following the manufacturers’ instructions.

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