2.2. Preparation of the nanoparticles by the antisolvent precipitation method

An exactly weighed quantity of 100 mg of FLZ was dissolved in 3 mL of dichloromethane. Thereafter, by using a syringe, the drug was dropped into distilled water (20 mL) containing the stabilizer (0.5, 1, 5 w/v %) under magnetic stirrer at 1000 rpm for 4 h until the solvent was completely evaporated, thereby resulting in supersaturation of the FLZ in solution and nanoparticle formation. The prepared FLZ nanosuspensions were frozen at −30 ^◦C and finally dried with a freeze-dryer (Martin Christ, Osterode, Germany) at −40 ^◦C (Ibrahim et al., 2019).