qPCR Analysis.

Total RNA was extracted using TriReagent (Ambion). First-stand cDNA was synthesized using iScript cDNA synthesis kit (BioRad, 170–8891). Relative gene expression or genome enrichment (i.e., ChIP-qPCR) was analyzed with iTaq universal SYBR Green (BioRad, 175–5124) using the CFX96 Real-Time PCR Detection system (Bio-Rad). Relative fold-changes were calculated after normalization to Gapdh levels using the 2^−∆∆Ct method. All qPCR experiments were repeated at least three times and representative experiments are presented. Error bars are SDs of triplicate qPCR reactions for the experiment shown. qPCR primers are listed in Dataset S4.