2.2. Llama Immunization and Phage Library Construction

Immunizations of llamas with various FMDV strains are summarized in Table S1. The immunizations of llamas 3049, 6058, 6666, and 7212 have been described previously [23,24]. Llama 9245 was intramuscularly immunized three times with a three-week interval with 50 μg 146S of A22/IRQ/24/64 and 50 μg 146S of O1/Manisa/TUR/69 each time, whereas llama 9246 received 50 μg 146S of A/TUR/14/98 according to the same schedule. In the fourth and seventh weeks post-immunization, the VHH repertoire of individual llamas was amplified by RT-PCR from peripheral blood lymphocytes and separately inserted into phage display vector pRL144 as described earlier [23,24].