Mouse strains

All animal experiments were approved by The Rockefeller University Institutional Animal Care and Use Committee. Mice were maintained in a C57BL/6N genetic background and housed in rooms on a 12-h dark/light cycle interval with food and water available ad libitum. GSAP conditional KO mice were constructed at Taconic Farms by targeting exons 9–11 of GSAP. Constitutive GKO mice were generated by crossing GSAP conditional KO mice with CMV-Cre mice. J20 mice were purchased from The Jackson Laboratory (B6.Cg-Zbtb20Tg(PDGFB-APPSwInd)20Lms/2Mmjax; Mutant Mouse Resource and Research Center stock no. 34836-JAX). Both male and female littermates obtained from in vitro fertilization were used for behavioral tests and sn-RNAseq.