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TRITC-phalloidin staining for detecting cytoskeletal reorganization
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SACC cells were fixed with 4% paraformaldehyde for 15 min, lysed with 0.2% Triton X-100 for 15 min and blocked with 5% BSA for 20 min. Immediately, cytoskeleton dye TRITC-phalloidin (Sigma) was incubated for 30 min and DAPI was used for nuclei staining. Images were visualized and captured using a fluorescence microscope.
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