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IAA concentration measurement using HPLC

SB Sakaoduoen Bunsangiam
NT Nutnaree Thongpae
SL Savitree Limtong
NS Nantana Srisuk
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The culture supernatant was collected by centrifugation for 5 min at 10000 × g and analyzed by an HPLC (Agilent Technologies, USA) equipped with a Cosmosil SC18-MS-II column (Nacalai Tesque, Japan) and UV detector (Agilent Technologies, USA) at 280 nm. The mobile phase contained solution A (methanol: acetic acid: water; 10:0.3:89.7 v/v/v) and 60% solution B (methanol: acetic acid: water; 90:0.3:9.7 v/v/v) at a flow rate of 0.3 mL/min as described by Nutaratat et al.24. Isocratic elution was used instead of gradient elution. Authentic IAA (Sigma, USA) was used as a standard.

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