Cell lines

HEK293T (ATCC CRL-3216) and C3H10T1/2 (ATCC CCL-226) were obtained from ATCC along with thorough reports of analysis confirming the cell line identification, authentication, and confirmed negative testing for mycoplasma contamination. The PPARγ cDNAs, including PPARγ2-WT and PPARγ2-2KR, were cloned into a doxycycline-inducible lentiviral plasmid, pTRIPZ (Thermo Open Biosystems) (Li et al., 2016), and were stably overexpressed into PPARγ^-/- mouse embryonic fibroblasts (MEFs) (Rosen et al., 2002) with a selection of puromycin (2.5 μg/mL). Cells were grown in high-glucose DMEM (Corning, 10-017) supplemented with 10% FBS (heat inactivated; Corning, 35-011-CV) and 1% penicillin/streptomycin. These PPARγ cell lines have been previously validated and tested (Kraakman et al., 2018).