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The undifferentiated PC12 cells (obtained from the Cell Bank at the Chinese Academy of Sciences, Shanghai, China) were cultured and maintained for 48 h in the differentiation medium (95% high-glucose DMEM, Gibco, USA) supplemented with 1% horse serum (HyClone, USA), 3% fetal bovine serum (Sijiqing, China), 1% penicillin-streptomycin (HyClone, USA) and 50 ng/mL nerve growth factor (R&D Systems, USA).
Copyright and License information: ©2021 The Author(s) 2021. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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