Croton test

Control animals received (1% Tween 80 in normal saline, 10 mL/Kg, i.p.) and test animals received either hydroalcoholic or hexane extract (100, 200, and 400 mg/kg i.p.). Indomethacin (10 mg/kg, i.p.) was injected as the standard drug. Thirty minutes later, 20 μL of croton oil solution was applied to the inner part of the animal's right ear. After 6 h, the animals were sacrificed and disc samples with 6 mm diameter were prepared from both ears. The discs were weighed and the difference between the weights of the left and right disks was reported as ear edema.[8]