Morris Water Maze Test

The MWM test was performed to assess spatial memory. In acquisition trial, gently place the mouse into the water, facing the edge of the pool. If the mouse finds the platform before the 90 s cut-off, allow the mouse to stay on the platform for 10 s then return it to its home cage. Otherwise, place the mouse on the platform and allow it to stay there for 20 s. The mouse was trained in different direction. Repeat for all mice in the trail in the next 3 days. In probe trial, remove the platform from the pool and the test time was 60 s. Escape latencies, time spent or distance traveled in the target quadrant and platform-crossing times were recorded and analyzed using the analysis management system (Beijing Zhongshi Kechuang CO., LTD).