2.7. Cells siRNA Transfection

SW480 cells were seeded 1 day in advance to ensure that the cell confluence reached 70%–80% before transfection. According to the instructions, Opti-MEM (Gibco, 31985-062, USA) was used to dilute RNAiMAX (Invitrogen, 13778-150, USA), SERPINH1-siRNA1, siRNA2, and siRNA3 (RiboBio, China), respectively. The diluted RNAiMAX was mixed with each siRNA dilution and the mixed dilution was added to the cell culture medium after standing still for 5 min. The final concentration of SERPINH1-siRNA1, siRNA2, and siRNA3 was 50 nM, respectively. After incubating at 37°C for 48 hours, the cells were harvested for subsequent experiments.