Validation of RNA-Seq Data by Quantitative Real-Time RT-PCR (qRT-PCR)

RNA samples were reverse transcribed into cDNA using a SuperScript III RT kit (Invitrogen, Carlsbad, CA, United States; Cat. No. 18080-044). The cDNA samples were subjected to qRT-PCR quantification with three biological replicates and performed with SYBR Green Master Mix (Applied Biosystems, Foster City, CA, United States; PN 4309155) according to the product manual on a CFX96 Real Time System (Bio-Rad, Hercules, CA, United States). Reference genes and specific gene primers are listed in Supplementary Table S1. The relative quantification method was used to evaluate the quantitative variation between the replicates examined.