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2.2. Assay of glutathione peroxidase activity

DO David C. Obasi
VO Victor N. Ogugua
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Gluthathione peroxidase (GPx) activity was assayed based on the method of Paglia and Valentine [14].

Principle: Gluthathione peroxidase catalyses the oxidation of glutathione (GSH) by colourless liquid hydrocarbon hydroperoxide. In the presence of glutathione reductase (GR) and NADPH, the oxidized glutathione (GSSG) is immediately converted to the reduced form with concomitant oxidation of NADPH to NADP+. The decrease in absorbance at 340 nm is measured.

GPx

GR

Procedure: A known volume of the sample (0.1 ml) was added into a test tube. Then, 0.1 ml of diluted reagent 1 (R1) was mixed with 0.1 ml of reagent 2 (R2) and was added into the test tube and mixed. The initial absorbance was taken after 30 s and the final absorbance was taken after 1 min at 340 nm. Glutathione peroxidase activity was calculated from the formula:

Copyright and License information:  ©2021 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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