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Using the cetyltrimethyl ammonium bromide method (Rogers and Bendich, 1988), DNA of fresh leaves was extracted after grinding in liquid nitrogen. Primers to amplify SSR sequences were synthesized based on sequence information (McCouch et al., 2002). PCR amplification, polyacrylamide denaturing gel electrophoresis, and silver-staining were performed as described previously (Panaud et al., 1996).
Copyright and License information: ©2017 Wang, Cao, Ma, Chen, Zhao, Sun, Tan and Liu.
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