Two different microfluidic chips were fabricated using soft lithography replica molding. One chip was used for microgel synthesis using emulsion polymerization and one for the filtration experiments. The positive master mold was 3D-constructed using Autodesk Inventor and fabricated by two-photon dip-in direct laser writing following the procedure described by Loelsberg et al.48,49. Afterward, PDMS (Sylgard 184, Farnell) was used to replicate the molds, holes for tube connection were punched, and the chip was bonded to a microscope slide with a thickness of 1 mm using oxygen plasma.
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