Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Phosphodiesterase enzyme selectivity assays
Ask a
question
Favorite
All 3′, 5′ cyclic nucleotide phosphodiesterase enzyme activities were measured with a radiometric enzyme assay based on scintillation proximity assay detection system or a filtration binding format. Compounds tested were diluted in pure DMSO using 10‐point concentration response curves. Selectivity against PDE3A (catalytic domain), PDE4 (A, B, C, and D), PDE7B, and PDE8A were measured using [3H]‐cAMP, whereas selectivity against PDE2A, PDE5A, PDE6AB, PDE9A, PDE10A, and PDE11A were measured using [3H]‐cGMP, as described. 5
Copyright and License information: ©2020 Eli Lilly and Company. published by Wiley Periodicals LLC on behalf of the American Society for Clinical Pharmacology and Therapeutics.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
