GraphPad Prism 7 software (GraphPad Prism, USA) was utilized for statistical analysis. Data are presented as mean ± standard deviation. By normality test, all data followed Gaussian distribution. Comparisons with different groups were analyzed using paired Student's t-test or one-way analyses of variance followed by Tukey's multiple comparison test. Differences with ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 were considered significant.
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