2.2. Decitabine treatment

RS Ryo Shimizu
TM Tomoya Muto
KA Kazumasa Aoyama
KC Kwangmin Choi
MT Masahiro Takeuchi
SK Shuhei Koide
NH Nagisa Hasegawa
YI Yusuke Isshiki
ET Emi Togasaki
CK Chika Kawajiri-Manako
YN Yuhei Nagao
ST Shokichi Tsukamoto
SS Shio Sakai
YT Yusuke Takeda
NM Naoya Mimura
CO Chikako Ohwada
ES Emiko Sakaida
TI Tohru Iseki
DS Daniel T. Starczynowski
AI Atsushi Iwama
KY Koutaro Yokote
CN Chiaki Nakaseko
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Human leukemia cell lines and primary AML cells were treated with different final concentrations of decitabine (DAC; Sigma-Aldrich, St Louis, MO, USA) or the same volume of phosphate buffered saline, replacing the medium and adding DAC or phosphate buffered saline every 24 h until harvested for RNA and DNA extraction.

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