Human leukemia cell lines and primary AML cells were treated with different final concentrations of decitabine (DAC; Sigma-Aldrich, St Louis, MO, USA) or the same volume of phosphate buffered saline, replacing the medium and adding DAC or phosphate buffered saline every 24 h until harvested for RNA and DNA extraction.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.