2.3. LPS-Induced ALI and Treatment

Mice were randomly divided into 7 groups, 5 in each group: control group, LPS group, LPS + BA − Mg (100, 200 mg/kg), LPS + BA (195.2 mg/kg), LPS + MgSO₄ (5.2 mg/kg), and LPS + DEX (5 mg/kg). The BA group and MgSO₄ group were administered with BA and MgSO₄ equivalent to 200 mg/kg BA-Mg, respectively. Briefly, all mice were pretreated with drugs intraperitoneally once each day for 3 days consecutively. On the fourth day, mice were anesthetized by 2.5% isoflurane inhalation and stimulated with 50 μL LPS (16 mg/mL) via dripping into the nasal cavity. Drugs were intraperitoneally injected 3 days post-LPS exposure. Mice were sacrificed at 1 h after the end of administration on the 6th day; then, bronchoalveolar lavage fluid (BALF) and lung tissues were obtained. All samples are stored at -80°C until assay.