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Metabolite Extraction and Metabolomic Profiling
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Mtb-laden filters used for metabolomic analyses were generated as previously described53 and incubated at 37 °C for 4 days to expand biomass. Mtb-laden filters were then transferred onto a fresh 7H10 agar plate with or without 1f and 2b at 5 and 50 μM for a further 24 h. Metabolite extraction and LC-MS-based metabolomic profiling were carried out as previously described.33 All data obtained by metabolomics were the average of independent triplicates.
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