Cell morphology studies of 5-FU loaded f-MWCNTs and 5-FU loaded f-SWCNTs by DAPI staining

Microplate covered with cover slip (24 well) having a flat bottom was selected to study the cell morphology. MCF-7 and COLO320DM cells were separately seeded in a plate, further; the plate was incubated in a CO₂ incubator with a 37.00 ± 1.00 °C temperature for an overnight. Subsequently, 100 μg mL⁻¹ of 5-FU loaded CNTs (MWCNTs and SWCNTs) solution was added into the labeled well. After the addition of CNTs, plate was again incubated for continuous 1 day (24 h) at 37.00 ± 1.00 °C. Then, the PBS solution was used for washing purpose and the resultant solution was fixed with 4% formaldehyde for half an hour. Further, 20 µL of DAPI staining solution was added in well containing CNTs and incubated for continuous 5 min at ambient temperature in the dark condition. DAPI treated samples of control, 5-FU loaded MWCNTs and 5-FU loaded SWCNTs were examined under fluorescent microscopy.