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Determination of TNFα, IL-1β and IL-6
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The cell culture supernatant was centrifuged in a 4° C centrifuge at 2000 rpm for 5 min, and the supernatant was collected and stored at -80° C for testing. The experiment was performed according to the instructions of the TNF-α, IL-1β and IL-6 detection kit. The absorbance value of the sample was measured, and the corresponding concentration was calculated according to the standard curve.
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