2.1. Cell Culture

E0771 medullary breast adenocarcinoma cells, originally isolated from a spontaneous mammary tumor in C57BL/6 mice, were obtained from Dr. Wakefield’s lab at the NIH, who in turn obtained them from Dr. Fengzhi Li in Dr. Enrico Mihich’s lab at Roswell Park Cancer Institute, Buffalo, NY. The E0771 stably expressing-Dendra2 were generated with standard lentiviral transfection procedures and were FACS sorted for the over-expression of Dendra2. Cancer cells were cultured in DMEM (cat #12320032, ThermoFisher, Grand Island, New York, USA) media supplemented with 10% (v/v) FBS (cat# S11550, R&D System, Flowery Branch, Ga, USA) and 1% penicillin/streptomycin (cat# 15140122, Gibco, Grand Island, NY, USA) and were routinely tested for mycoplasma (LookOut Mycoplasma PCR detection kit, cat #MO0035-1KT, Sigma, St. Louis, MO, USA).