2.5. Critical Micelle Concentration Measurement

Critical micelle concentration (CMC) was determined in accordance with previously reported methods [34]. The dilutions of 0.05–100 mg/L copolymers containing a fixed amount of pyrene were analysed via fluorescence spectroscopy. The excitation spectra of Py from 300 to 360 nm was monitored at an emission wavelength of 390 nm by using a Cary Eclipse fluorescence spectrometer (Agilent, Santa Clara, CA, USA).