4.6. Cell Viability Assay

An MTS Assay Kit (Abcam, ab197010) was used for measurements of cell viability. Cells were seeded into 96-well plates at 5000 cells/well and incubated overnight. After treatment with SMPs for 48 and 72 h, MTS reagent was added to the cell culture media and incubated for 1.5 h at 37 °C. Finally, the optical density (OD) of the solution was determined with a spectrometer at a wavelength of 490 nm. All frig-treated cells were normalized with their respective untreated control cells, which were considered as 100% viable. Data were expressed as mean ± SD of triplicate experiments. IC50 values were calculated with GraphPad Prism v8.3.0 (GraphPad Inc., La Jolla, CA, USA).