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2.5. Isolation and Reverse Transcription of miRNAs from Mammalian Cells
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The isolation and purification of miRNAs were performed using the miRNeasy Mini Kit from Qiagen (Hilden, Germany) according to the manufacturer. For the reverse transcription, the miScript II RT Kit from Qiagen (Hilden Germany) was used. In this study, 500 ng of miRNA were reversed transcribed into micDNA according to the manufacturer’s specification.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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