ReHo Value Analysis

Group analysis was performed with a random effects model using SPM12. We first compared the difference in ReHo between patients with MwoA and HCs using two-sample t-test. We then compared the changes in ReHo in different stages during acupuncture treatment using one-way ANOVA within subjects; post-hoc comparisons were used to test the changes in ReHo between different periods. To explore the association between clinical outcomes and the ReHo value, we extracted the Z-values for each patient in the regions of interest, in which patients with MwoA showed a decreased ReHo value before acupuncture when compared with HCs and a significantly increased ReHo value after acupuncture, and used Pearson/Spearman correlation to explore the association between these brain regions and the corresponding migraine symptoms. A threshold of family-wise error (FWE) corrected P-value of <0.05 at the voxel level or at the cluster level were applied for all analyses.