CTLL-2 cell proliferation assay

HX Huan Xu
MS Mingyang Shi
CS Changsheng Shao
HL Hao Li
JW Jing Wu
YY Yin Yu
FF Fang Fang
YG Yugang Guo
WX Weihua Xiao
request Request a Protocol
ask Ask a question
Favorite

A CTLL-2 cell proliferation assay was used to determine the biological activity of the rhIL-15 and IL-15/SuIL-15Rα-IgG4 Fc complexes. In brief, CTLL-2 cells in logarithmic growth phase were washed thrice with RPMI medium containing 10% FBS (basal medium) and then seeded into 96-well culture plates at 1 × 104 cells per well (60 μL). The rhIL-15 and IL-15/SuIL-15Rα-IgG4 Fc complexes were serially diluted with basal medium and added into the plates at 40 μL per well. After 48 h of incubation, the cell viability was measured using the MTT colorimetric assay. The results were analyzed by GraphPad Prism software, and EC50 values were calculated using the four-parameter fit logistic equation.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A